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. Author manuscript; available in PMC: 2024 Dec 27.
Published in final edited form as: Adv Healthc Mater. 2024 Sep 19;13(32):e2402199. doi: 10.1002/adhm.202402199

Figure 4. Characterization for differentiation of hSCOs in different HAMA hydrogels.

Figure 4.

(A) Flow cytometry analysis of expression of different ventral markers when generating hSCOs in different hydrogels. (B) Summary of 3 runs of flow cytometry analysis for identification ventral hSCO marker expression. (C) RT-PCR analysis of relative mRNA expression for different region-specific patterning markers during generation of hSCOs at day 35 (n=3). * indicates p≤0.05, **: p≤0.01, ***: p≤0.001. (D) (i) Electrophysiology to show sodium and potassium currents for the replated hSCOs at day 40. (ii) Morphology of outgrowth cells of the replated hSCOs for electrophysiology. Scale bar = 20 μm.