REAGENT	SOURCE	IDENTIFIER
Goat anti‐rabbit RDye® 680	LI‐COR Biosciences	Cat#926‐32221; RRID: AB_621841
Goat anti‐mouse RDye ® 800CW	LI‐COR Biosciences	Cat#926‐32210; RRID: AB_621842
Goat anti‐rabbit IgG Chromeo 546	Abcam	Cat#ab60317; RRID: AB_954976
Goat anti‐rabbit IgG Chromeo 488	Abcam	Cat#ab60314; RRID: AB_954969
Alexa Flour 555 donkey anti‐mouse	Invitrogen	Cat#A31570; RRID: AB_2536180
Chemicals, peptides, and recombinant proteins
GMEM	Gibco	Cat#11710035
DMEM	Gibco	Cat#11965092
N2	Gibco	Cat#17502001
B27	Gibco	Cat#17504044
DAPI	Sigma	Cat#D9542
CHIR99021	Qcbio Science & Technologies	Cat#04‐0004
PD0325901	Qcbio Science & Technologies	Cat#04‐0006
AG490	Beyotime	Cat# S1509
Trichostatin A	Beyotime	Cat# S1893
Nocodazole	Beyotime	Cat# S1765
LY294002	Beyotime	Cat# S1737
Monensin	Beyotime	Cat# S1753
Nicotinamide	Beyotime	Cat# S1761
5‐(N,N‐Dimethy)amiloride hydrochloride (DMA)	Sigma	Cat# A4562
ESGRO® mLIF	Millipore	Cat# ESG1107
recombinant human LIF	Millipore	Cat# LIF1005
recombinant mouse LIF	Millipore	Cat# LIF2010
Critical commercial assays
JC‐1 kit	Beyotime	Cat# C2006
Lyso‐Tracker Red	Beyotime	Cat# C1046
Nuclear protein extraction	Beyotime	Cat#P0028
c‐Myc Tag IP/Co‐IP kit	Thermo Scientific	Cat#23625
QuikChange® XL Site‐directed mutagenesis kit	Stratagene	Cat#200517
Cycle TESTTM PLUS kit	Becton Dickinson	Cat#340242
Prime Script kit	Takara	Cat#RR014A
Wright's staining solution	Nanjing Jiancheng Bioengineering Institute	Cat#D007
Mouse Regulatory T Cell Staining Kit	MultiSciences Biotech Co., Ltd	Cat#KTR201
Mouse Th17 Staining Kit	MultiSciences Biotech Co., Ltd	Cat#KTH217
Deposited data
The mice embryo of STAT3 K177/180R RNA‐seq	This paper	GSE271656