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. 2024 Nov 23;43(12):115001. doi: 10.1016/j.celrep.2024.115001

Figure 4.

Figure 4

Pressure overload-induced cardiac dysfunction in Bcl2-L-13 (S272A) knockin mice

(A) Western blot analysis of phospho- Bcl2-L-13 (Ser272) in the Bcl2l13+/+ (WT) and Bcl2l13−/− (KO) mouse hearts 4 weeks after TAC (n = 3). The bar graph shows densitometric analysis.

(B) Representative images of M-mode echocardiographic tracings from sham- or TAC-operated wild-type (WT) or Bcl2-L-13 (S272A) knockin (KI) mice 4 weeks after surgery. Scale bars, 0.1 s and 2 mm.

(C) Echocardiographic parameters (n = 10).

(D) Physiological parameters (n = 10).

(E) mRNA expression of Nppa and Nppb (n = 8). Gapdh mRNA was used as the loading control. The average value in the WT sham group was set to 1.

(F) Wheat germ agglutinin-stained heart sections. Scale bar: 50 μm. Cardiomyocyte cross-sectional areas were measured by tracing the outline of 100 myocytes in a non-fibrotic area in each section (n = 4). Results are shown as mean with 95% CI. Statistical analysis by one-way ANOVA followed by Tukey-Kramer’s post hoc test. All pairwise comparisons were performed. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figure S4.