| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal Anti-HA tag (clone C29F4) | Cell Signaling Technology | Cat# 3724; RRID: AB_1549585 |
| Rabbit polyclonal anti-LC3B | Cell Signaling Technology | Cat# 2775; RRID: AB_915950 |
| Mouse monoclonal anti-Myc Tag (clone 9B11) | Cell Signaling Technology | Cat# 2776; RRID: AB_390779 |
| Rabbit polyclonal anti-Phospho-DRP1 (Ser637) | Cell Signaling Technology | Cat#4867; RRID: AB_10622027 |
| Rabbit polyclonal anti-Phospho-DRP1 (Ser616) | Cell Signaling Technology | Cat# 3455; RRID: AB_2085352 |
| Rabbit monoclonal anti-Mitofusin2 (clone D2D10) | Cell Signaling Technology | Cat# 9482; RRID: AB_2716838 |
| Rabbit monoclonal anti-Mitofusin1 (clone D6E2S) | Cell Signaling Technology | Cat# 14739; RRID: AB_2744531 |
| Rabbit monoclonal anti-OPA1 (clone D6U6N) | Cell Signaling Technology | Cat# 80471; RRID: AB_2734117 |
| Mouse monoclonal anti-GAPDH (clone D4C6R) | Cell Signaling Technology | Cat# 97166; RRID: AB_2756824 |
| Mouse monoclonal anti-α-Tubulin (clone DM1A) | Cell Signaling Technology | Cat# 3873; RRID: AB_1904178 |
| Rabbit polyclonal anti-Parkin | Cell Signaling Technology | Cat# 2132; RRID: AB_10693040 |
| Rabbit monoclonal anti-PINK1 (clone D8G3) | Cell Signaling Technology | Cat# 6946; RRID: AB_11179069 |
| Rabbit polyclonal anti-AMPKα1 | Cell Signaling Technology | Cat# 2795; RRID: AB_560856 |
| Rabbit polyclonal anti-AMPKα2 | Cell Signaling Technology | Cat# 2757; RRID: AB_560858 |
| Rabbit polyclonal anati-AMPKα2 | Proteintech | Cat# 18167-1-AP; RRID: AB_10695046 |
| Rabbit polyclonal anti-BCL2L13 | Proteintech | Cat# 16612-1-AP; RRID: AB_1850928 |
| Mouse monoclonal anti-PGC1-α (1C1B2) | Proteintech | Cat# 66369-1-Ig; RRID: AB_2828002 |
| Mouse monoclonal anti-FLAG tag (clone M2) | Sigma-Aldrich | Cat# F1804; RRID: AB_262044 |
| Mouse monoclonal anti-α-sarcomeric actin | Sigma-Aldrich | Cat# A2172; RRID: AB_476695 |
| Rabbit polyclonal anti-TTC11 | abcam | Cat# ab96764; RRID: AB_10679033 |
| Rabbit polyclonal anti-HMGB1 | abcam | Cat# ab18256; RRID:AB_444360 |
| Mouse monoclonal anti-ATP synthase | Life Technologies | Cat# A-21351; RRID: AB_221512 |
| Mouse monoclonal anti-Drp1 (clone 22) | BD Transduction Laboratories | Cat# 611738; RRID: AB_399214 |
| Sheep Anti-Mouse IgG HRP Conjugated | Amersham | Cat#NA931; RRID: AB_772210 |
| Donkey Anti-Rabbit IgG HRP Conjugated | Amersham | Cat# NA934; RRID: AB_772206 |
| IRDye 800CW Donkey anti-Goat IgG | LI-COR Biosciences | Cat# 926–32214; RRID: AB_621846 |
| IRDye 680LT Goat anti-Mouse IgG | LI-COR Biosciences | Cat# 926–68020; RRID: AB_10706161 |
| IRDye 680RD Donkey anti-Rabbit IgG | LI-COR Biosciences | Cat# 926–68073; RRID: AB_10954442 |
| IRDye 800CW Donkey anti-Mouse IgG | LI-COR Biosciences | Cat# 926–32212; RRID: AB_621847 |
| IRDye 800CW Goat anti-Rabbit IgG | LI-COR Biosciences | Cat# 926–32211; RRID: AB_621843 |
| Goat anti-Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Thermo Fisher Scientific | Cat# A-21043; RRID:AB_2535712 |
| Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 | Thermo Fisher Scientific | Cat# A-11034; RRID:AB_2576217 |
| Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Thermo Fisher Scientific | Cat# A-11057; RRID:AB_2534104 |
| Chicken Anti-Goat IgG (H + L) Antibody, Alexa Fluor 647 Conjugated | Thermo Fisher Scientific | Cat# A21469; RRID:AB_10374877 |
| Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | Thermo Fisher Scientific | Cat# A10037; RRID:AB_11180865 |
| Bacterial and virus strains | ||
| Genlantis TurboCells BL21Competent Cells | Gelantis | AMS.C302020 |
| Chemicals, peptides, and recombinant proteins | ||
| RNAi MAX | Invitrogen | Cat# 13778030 |
| ScreenFect A | Wako | Cat# 299-73203 |
| Penicillin-Streptomycin-Glutamine | Gibco | Cat# 10378016 |
| geneticin | Gibco | Cat# 10131-027; CAS: 108321-42-2 |
| Bafilomycin A1 | LC Laboratories | Cat# B-1080; CAS: 88899-55-2 |
| ECL Prime Western Blotting Detection Reagent | GE Healthcare Life Science | Cat# RPN2232 |
| Lumigen ECL Ultra | Lumigen | Cat# TMA-100 |
| CCCP | Sigma-Aldrich | Cat# C2759; CAS: 555-60-2 |
| ProLong Gold Antifade Mountant with DAPI | Thermo Fisher Scientific | Cat# P36935 |
| MitoSox | Life Technologies | Cat# M36008 |
| fluorescein isothiocyanate-conjugated lectin | Sigma | L4895 |
| protease inhibitor cocktail | Cell Signaling Technology | #5871 |
| phosphatase inhibitor cocktail | Cell Signaling Technology | #5870 |
| Dynabeads Protein A for Immunoprecipitation | Thermo Fisher Scientific | Cat# 1001D |
| GST Spin Purification Kit | Thermo Scientific Pierce | Cat# 11804025 |
| PreScission Protease | GE Healthcare Lifescience | – |
| AMPK A2/B1/G1 Recombinant Human Protein | Thermo Fisher Scientific | Cat# PV4674 |
| CAMK2B (CaMKII Beta) Recombinant Human Protein | Thermo Fisher Scientific | Cat# PV4205 |
| ERK4 Recombinant Protein | SignalChem | Cat# M30-34G-20 |
| FASTK Recombinant Protein | SignalChem | Cat# F01-10G-05 |
| Haspin Recombinant Protein | SignalChem | Cat# G10-11G-05 |
| IRAK2 Recombinant Protein | SignalChem | Cat# I10-10BG-05 |
| NLK Recombinant Human Protein | Thermo Fisher Scientific | Cat# PV4309 |
| CDC42 BPA (MRCKA) Recombinant Human Protein | Thermo Fisher Scientific | Cat# PV4398 |
| Recombinant human PKM2 protein | abcam | Cat# ab89364 |
| MAP3K7/MAP3K7IP1 (TAK1-TAB1) Recombinant Human Protein | Thermo Fisher Scientific | Cat# PV4394 |
| ACVR2A Human Protein | Thermo Fisher Scientific | Cat# PV6124 |
| AAK1 Recombinant Human Protein | Thermo Fisher Scientific | Cat# A30967 |
| RIPK3 Recombinant Protein | SignalChem | Cat# R09-10G-05 |
| PKAc beta, active | Eurofins Pharma Discovery | Cat# 15-007 |
| BAPTA-AM | TCI chemicals | Cat# T2845 |
| Dorsomorphin | FUJIFILM Wako | Cat# 044-33751 |
| Quercetin | Sigma-Aldrich | Cat# Q4951 |
| N-acetylcysteine | Sigma-Aldrich | Cat# A9165: CAS: 616-91-1 |
| Critical commercial assays | ||
| ATP Assay Kit | abcam | Cat# ab83355 |
| In situ Apoptosis Detection Kit | Takara Bio | Cat# MK500 |
| AMPK Kinase Assay Kit | Cyclex | Cat# CY-1182 |
| DNeasy Blood & Tissue Kit | QIAGEN | Cat# 69504 |
| mouse Real-time PCR Mitochondrial DNA Damage Analysis Kit | Detroit R&D | Cat# DD2M |
| Deposited data | ||
| Raw and analyzed data | This paper; Figshare | Tables S5, S6 and S7; https://doi.org/10.6084/m9.fig.share.25284883 |
| Images from primary and second screening | This paper; Dryad | https://doi.org/10.5061/dryad.9cnp5hqsx |
| Experimental models: Cell lines | ||
| Human: HEK293A Cell line | Invitrogen | Cat# R70507; RRID: CVCL_6910 |
| Experimental models: Organisms/strains | ||
| Bcl2-L-13 knockout mice | This paper | N/A |
| Bcl2-L-13 S272A knockin mice | This paper | N/A |
| Oligonucleotides | ||
| Silencer™ Human Kinase siRNA Library | ThermoFisher Scientific | Cat# A30079 |
| siRNA: AMPKα1 | ThermoFisher Scientific | Cat# AM51551, siRNA ID: 768 |
| siRNA: AMPKα2 | ThermoFisher Scientific | Cat# AM51331, siRNA ID: 771 |
| Negative Control No. 1 siRNA | ThermoFisher Scientific | Cat# 4390843 |
| Recombinant DNA | ||
| pDONR223-PRKAA2 | Johannessen et al.35 | Addgene; Cat# 23671 |
| HA-Bcl2-L-13-pcDNA3.1 | Murakawa et al.8 | N/A |
| myc-AMPKα2-pcDNA3.1 | This paper | N/A |
| pGEX-Bcl2-L-13 | This paper | N/A |
| Software and algorithms | ||
| ImageJ (Version 1.52p) | National Institue of Health, USA | https://imagej.nih.gov/ij/index.html |
| Image Studio | LI-COR Biosciences | N/A |
| ImageQuantTL v7.0 | GE Healthcare Life Sciences | – |
| Graphpad Prism 9 | GraphPad Software | N/A |
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