Figure 2.

Influence of EVs on invasion of astrocytes, GBM 011, and U251 MG cells. (a) Representative images showing the cells migrated through 8 µM pore to the bottom of the migration chamber upon incubation with EVs (×100 magnification, scale bar = 100 μm); (b) number of the invaded cells. Data are shown as the number of invaded cells upon incubation with EVs normalized to the number of invaded untreated cells (100%, dashed line) ± SEM (n = 8); && (p < 0.01) indicates significant difference from the untreated cells according to one-sample t-test followed by post hoc Holm–Sidak’s test; $ (p < 0.05) indicates significant difference from the untreated cells according to one-sample Wilcoxon test, followed by post hoc Holm–Sidak’s test; * (p < 0.05) and *** (p < 0.001) indicate significant difference between the data groups according to one-way ANOVA followed by post hoc Tukey’s test; # (p < 0.05) and ### (p < 0.001) indicate significant difference between the data groups according to Kruskal–Wallis test followed by post hoc Dunn’s test.