Table 1.

Antibiotics used for ribosome engineering of M. xanthus ZE9

Antibiotics	Targets	Mutant gene	MIC (µg/mL)	Screening concentration (µg/mL)	Mutant rate
Streptomycin	30S	rpsL or rsmG	> 256	–	–
Gentamicin	30S	rplF	> 256	–	–
Spectinomycin	30S	–	> 256	–	–
Paromomycin	30S	rpsL	64	200	6 × 10–7
Rifampicin	β subunit of RNA polymerase	rpoB	1	2	3 × 10–8
Neomycin	30S	–	64	150	1 × 10–8
Tetracycline	30S	–	8	10	1 × 10–7