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(a) Fluorescence imaging of exogenous ONOO− (0, 2, 4, 6, 8, and 10 μM) in HeLa cells stained with MOBDP-I (10 μM). scale bar: 50 μm. (b) Relative fluorescence intensities corresponding to panel (a) in both the green and red channels. (c) Fluorescence intensity ratio corresponding to panel (a). Green channel: λex = 488 nm, λem = 510–540 nm. Red channel: λex = 543 nm, λem = 580–650 nm. The error bars represent ± standard deviation (SD) (n = 3).
