Table 1.

Summary of study characteristics

Author	Year	Country	Number of patients	Diagnostic methods	Sample type	TP	FP	FN	TN	Type of research	Sequencing methods	Sequencing conditions
Zhou X [19]	2019	China	105	Mycobacteria culture Xpert mNGS Xpert and mNGS	BALF/sputum/lung biopsy tissue	7/1	0/0	4/1	11/2	Prospective	DNA/ RNA-Seq	BGISEQ
Jin W T [17]	2020	China	820	Mycobacteria culture mNGS	BALF/ sputum/ lung biopsy tissue	11/12/8	2/4/1	10/11/1	108/265/44	Retrospective	DNA-Seq	BGISEQ
Shi C L [20]	2020	China	110	Mycobacteria culture Xpert mNGS Xpert and mNGS	BALF/sputum	23	1	25	61	Prospective	DNA-Seq	Others
Chen P X [16]	2020	China	70	Mycobacteria culture Xpert mNGS	BALF/sputum/lung tissue	11/3	0/0	3/0	14/3	Prospective	DNA-Seq	BGISEQ
Liu X [21]	2020	China	311	Mycobacteria culture AFB Xpert mNGS	BALF	118	28	93	83	Retrospective	DNA-Seq	BGISEQ
Zhu N [22]	2021	China	107	Mycobacteria culture AFB T-SPOT mNGS	BALF/ lung biopsy tissue	29/12	1/1	3/2	45/14	Retrospective	DNA-Seq	BGISEQ
Jin X [24]	2022	China	246	Mycobacteria culture AFB T-SPOT Xpert mNGS	BALF	19	99	9	119	Retrospective	DNA/ RNA-Seq	BGISEQ
Xu P [25]	2022	China	94	AFB T-SPOT Xpert mNGS	BALF/Lung biopsy specimens	67	0	4	23	Retrospective	DNA-Seq	Others
Fu M [23]	2022	China	403	Sputum stain PPD test CT Xpert mNGS	BALF/ lung biopsy tissue	21/15	0/0	15/21	21/15	Retrospective	DNA-Seq	Others
Gao J [26]	2023	China	186	Mycobacteria culture Xpert mNGS AFB	BALF	30	0	8	148	Retrospective	DNA -Seq	BGISEQ
Zhang D [29]	2023	China	217	Mycobacteria culture Xpert mNGS AFB ddPCR	Total lung specimens	86	0	14	117	Retrospective	DNA -Seq	Others
Hao J [27]	2023	China	266	Mycobacteria culture mNGS AFB T-SPOT Smear	BALF	40	3	10	112	Prospective	DNA -Seq	Others
Liu Y [28]	2023	China	52	mNGS AFB T-SPOT	Total lung specimens	20	8	0	13	Retrospective	DNA -Seq	Others

Total lung specimens including BALF and/or lung biopsy tissue and/or sputum

CT computerized tomography, PPD positive purified protein derivative, AFB acid-fast bacilli