Table 2.
Chemical | Murine LLNA Category | h-CLAT Judgment | GAPDH | HMOX1 | JUN | PPP1R15A | BTG2 | DDIT3 | EGR1 | ||||||||||||
NGS | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | |||
2,4-Dinitrochlorobenzene | Extreme | p | 1.2 | 5.2 | 3.2 | 7.7 | 9.3 | 9.3 | 8.0 | 3.3 | 30.7 | 1.6 | 1.4 | 5.4 | 8.3 | ||||||
3.2 | Match | 10.0 | Match | 21.1 | Match | 4.3 | Match | 0.7 | Match | 8.1 | Match | ||||||||||
2.9 | 10.0 | 5.9 | 2.8 | 1.4 | 8.3 | ||||||||||||||||
1,4-Phenylendiamine | Strong | p | 0.9 | 83.7 | 66.3 | 27.2 | 19.2 | 25.6 | 12.1 | 10.1 | 15.7 | 6.1 | 5.1 | 5.1 | 14.9 | ||||||
69.6 | Match | 22.8 | Match | 19.2 | Match | 16.7 | Match | 5.5 | Match | 16.4 | Mismatch | ||||||||||
61.0 | 19.2 | 38.9 | 28.8 | 9.6 | 15.3 | ||||||||||||||||
Nickel sulfate | Moderate | p | 1.1 | 3.4 | 2.3 | 4.5 | 2.8 | 3.1 | 2.2 | 4.6 | 3.9 | 2.4 | 1.5 | 1.1 | 1.3 | ||||||
2.3 | Match | 3.0 | Match | 2.0 | Match | 6.8 | Match | 2.2 | Match | 1.1 | Match | ||||||||||
2.6 | 3.1 | 4.8 | 5.4 | 3.9 | 1.0 | ||||||||||||||||
2-Mercaptobenzothiazole | Moderate | p | 1.1 | 63.7 | 39.7 | 25.1 | 27.6 | 12.3 | 10.1 | 3.8 | 9.3 | 27.2 | 33.4 | 3.2 | 3.9 | ||||||
37.3 | Match | 24.9 | Match | 27.3 | Match | 6.1 | Match | 11.6 | Mismatch | 3.6 | Match | ||||||||||
22.5 | 25.4 | 21.7 | 5.2 | 63.1 | 3.4 | ||||||||||||||||
R(+)-Limonene | Weak | p | 1.1 | 61.5 | 62.2 | 28.1 | 54.2 | 9.1 | 10.1 | 3.5 | 11.1 | 8.6 | 9.8 | 18.6 | 50.9 | ||||||
46.9 | Match | 54.9 | Match | 29.0 | Match | 4.6 | Match | 7.4 | Match | 36.5 | Match | ||||||||||
54.6 | 63.6 | 17.8 | 6.1 | 18.8 | 32.9 | ||||||||||||||||
Imidazolidinyl urea | Weak | p | 1.2 | 7.4 | 3.9 | 80.0 | 65.3 | 15.6 | 10.9 | 13.5 | 15.8 | 2.5 | 2.2 | 8.3 | 10.9 | ||||||
4.1 | Match | 46.8 | Match | 13.2 | Match | 18.6 | Match | 4.6 | Match | 8.3 | Match | ||||||||||
4.2 | 51.9 | 17.9 | 24.1 | 3.3 | 8.6 | ||||||||||||||||
Isopropanol | non-sensitizer | n | 1.0 | 1.3 | 1.6 | 1.4 | 0.8 | 1.2 | 1.1 | 1.4 | 1.6 | 1.7 | 2.3 | 0.6 | 0.8 | ||||||
1.5 | Match | 0.7 | Match | 0.9 | Match | 1.5 | Match | 3.3 | Match | 0.6 | Match | ||||||||||
1.5 | 0.9 | 1.8 | 1.6 | 3.7 | 0.5 | ||||||||||||||||
Glycerol | non-sensitizer | n | 1.0 | 1.5 | 1.4 | 1.2 | 0.8 | 1.0 | 1.0 | 0.9 | 1.4 | 1.1 | 1.1 | 1.1 | 1.6 | ||||||
1.6 | Match | 1.2 | Match | 1.0 | Match | 1.3 | Match | 1.2 | Match | 1.0 | Match | ||||||||||
0.9 | 0.8 | 1.1 | 0.8 | 1.6 | 1.0 | ||||||||||||||||
4-Aminobenzoic acid | non-sensitizer | n | 1.1 | 1.3 | 0.8 | 0.6 | 0.8 | 1.0 | 0.8 | 1.4 | 1.7 | 0.9 | 0.8 | 0.5 | 0.8 | ||||||
0.8 | Match | 0.6 | Match | 2.0 | Match | 1.1 | Match | 0.3 | Match | 0.5 | Match | ||||||||||
0.9 | 0.8 | 0.9 | 4.5 | 1.2 | 0.6 | ||||||||||||||||
Chemical | Murine LLNA Category | h-CLAT Judgment | GAPDH | GADD45B | PMAIP1 | SAT1 | ULBP2 | CCR2 | ICMT | ||||||||||||
NGS | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | NGS | PCR | Judgment | |||
2,4-Dinitrochlorobenzene | Extreme | p | 1.2 | 7.3 | 8.1 | 7.7 | 6.9 | 2.4 | 0.5 | 7.1 | 1.6 | 0.1 | 0.2 | 0.5 | 0.5 | ||||||
7.4 | Match | 6.5 | Match | 16.6 | Mismatch | 8.5 | Match | 0.3 | Mismatch | 0.5 | Match | ||||||||||
5.8 | 9.3 | 1.7 | 4.6 | 0.2 | 0.7 | ||||||||||||||||
1,4-Phenylendiamine | Strong | p | 0.9 | 14.6 | 18.3 | 5.6 | 4.3 | 16.5 | 8.4 | 8.9 | 3.7 | 0.0 | 0.1 | 0.3 | 0.3 | ||||||
12.1 | Match | 2.9 | Match | 14.4 | Match | 1.6 | Mismatch | 0.0 | Match | 0.5 | Match | ||||||||||
25.6 | 9.9 | 24.1 | 12.6 | 0.0 | 0.4 | ||||||||||||||||
Nickel sulfate | Moderate | p | 1.1 | 0.9 | 1.0 | 3.6 | 2.8 | 2.3 | 1.5 | 1.4 | 0.5 | 0.6 | 0.6 | 0.6 | 0.6 | ||||||
0.7 | Match | 2.6 | Match | 2.0 | Match | 0.3 | Mismatch | 0.5 | Match | 0.8 | Match | ||||||||||
1.4 | 4.3 | 3.5 | 1.8 | 0.7 | 0.8 | ||||||||||||||||
2-Mercaptobenzothiazole | Moderate | p | 1.1 | 6.5 | 7.9 | 4.5 | 4.9 | 12.7 | 2.2 | 4.2 | 0.9 | 0.1 | 0.1 | 0.4 | 0.6 | ||||||
7.2 | Match | 3.9 | Match | 27.3 | Mismatch | 4.6 | Mismatch | 0.1 | Match | 0.6 | Match | ||||||||||
8.6 | 6.5 | 27.7 | 8.6 | 0.2 | 0.6 | ||||||||||||||||
R(+)-Limonene | Weak | p | 1.1 | 3.4 | 3.5 | 3.9 | 3.9 | 8.0 | 3.6 | 7.5 | 2.2 | 0.1 | 0.5 | 0.2 | 0.5 | ||||||
3.5 | Match | 4.2 | Match | 44.6 | Mismatch | 11.6 | Mismatch | 0.3 | Mismatch | 0.9 | Mismatch | ||||||||||
3.4 | 8.3 | 17.9 | 17.6 | 0.3 | 0.7 | ||||||||||||||||
Imidazolidinyl urea | Weak | p | 1.2 | 12.0 | 6.0 | 19.4 | 14.5 | 15.0 | 12.3 | 24.3 | 21.3 | 0.0 | 0.0 | 0.1 | 0.2 | ||||||
5.9 | Mismatch | 15.8 | Match | 18.5 | Match | 11.8 | Match | 0.0 | Match | 0.2 | Mismatch | ||||||||||
9.3 | 32.7 | 13.1 | 26.7 | 0.0 | 0.2 | ||||||||||||||||
Isopropanol | non-sensitizer | n | 1.0 | 1.2 | 1.4 | 1.2 | 2.1 | 1.3 | 2.1 | 1.1 | 1.6 | 0.8 | 1.0 | 1.0 | 0.9 | ||||||
0.7 | Match | 2.2 | Match | 3.7 | Mismatch | 0.8 | Match | 0.7 | Match | 1.2 | Match | ||||||||||
1.9 | 2.1 | 2.8 | 3.0 | 1.2 | 1.1 | ||||||||||||||||
Glycerol | non-sensitizer | n | 1.0 | 0.9 | 1.1 | 1.1 | 1.3 | 1.0 | 1.0 | 0.8 | 0.9 | 1.2 | 1.8 | 0.9 | 0.7 | ||||||
0.7 | Match | 1.0 | Match | 1.5 | Match | 0.4 | Match | 1.1 | Match | 1.5 | Match | ||||||||||
1.1 | 2.0 | 1.9 | 2.1 | 1.1 | 1.1 | ||||||||||||||||
4-Aminobenzoic acid | non-sensitizer | n | 1.1 | 1.1 | 1.0 | 1.3 | 0.9 | 1.1 | 0.3 | 1.6 | 0.4 | 0.7 | 0.6 | 0.9 | 3.3 | ||||||
0.7 | Match | 0.8 | Match | 3.8 | Match | 1.2 | Match | 0.8 | Match | 0.9 | Match | ||||||||||
0.6 | 8.2 | 1.8 | 2.1 | 0.5 | 1.0 |
The potency of the sensitizers in the murine LLNA [3,19] is classified as extreme, strong, moderate or weak. In the judgment by h-CLAT [7,26], if either CD86 or CD54 was positive, the test chemical is also judged as positive, “p”. Otherwise, it is indicated by “n”. If the results of new generation sequencing (NGS) and real-time PCR are consistent, it is marked as “Match”; if not, it is marked as “Mismatch”.