Figure 1.
Partial alignment resulting from the sequencing of the PDS genes of the banana genotypes Bucaneiro (AA), Zebrina (AA), and Prata-Anã (AAB) and sequences of the PDS gene of Musa acuminata and Musa balbisiana for gRNA design. The blue arrows indicate the selected gRNAs. Yellow highlights represent the gRNA nucleotides and green highlights indicate the PAM sequences. * Correspondence between the compared sequences, indicating a high degree of conservation.