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. 2024 Dec 20;46(12):14422–14437. doi: 10.3390/cimb46120865

Figure 3.

Figure 3

Digestion of the parts and vectors of interest in Gibson Assembly. M: 1 kB Plus NeoBio molecular weight marker (CV-1000 kB); 1: miniprep from Part 1 (undigested control); 2: 699 bp fragment excised from Part 1; 3: miniprep from Part 2 (undigested control); 4 and 5: 697 bp fragments excised from Part 2; 6: Cas9 miniprep in pUC57 (undigested control); 7 and 8: 5115 bp fragments excised from Cas9; 9: pDIRECT-22C vector miniprep (undigested control); 10, 11, and 12: 5400 bp fragments excised from pDIRECT-22C.