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PCR amplification of the Agrobacterium tumefaciens strain transformed with constructs 1 and 2. (A) PCR with primer set VC9_Fw and VC9_Rv (M: molecular weight marker 1 kB Plus DNA Ladder—MMK-130S (Cellco); C(-): negative control of the reaction with water; 1 and 2: 1912 bp amplicons at 45 °C for constructs 1 and 2). (B) PCR for primers Vp3C9_Fw and Vp3C9_Rv; M: marker; C(-): negative control; 1 and 2: 1000 bp amplicons at 51 °C.
