Figure 6.
Process of genetic transformation and regeneration of the Prata-Anã banana. (A) Embryogenic cell suspension used for transformation with the CRISPR/Cas9 plasmid; (B) embryos in 2,4-D culture medium and transforming Agrobacterium; (C) embryos in polyester membrane and 2,4-D + AS culture medium (T1) after 0 days and the different treatments (D–G) with BAP + AIA + Kan + Timentin culture medium ((D): T1, (E): T2, (F): T3, (G): T4) after 70 days of transformation, kept at 25 °C with a 16 h/8 h photoperiod.