Table 1.
Composition of the parts used in constructing the CRISPR/Cas 35S vectors and the specific banana root promoter.
| Parts | Construction of CRISPR/Cas9 Vectors CRISPR/Cas9 |
|---|---|
| Composition | |
| Part 1 | LB_U6 promoter_gRNA BsaI_U6 gene termination signal |
| Part 2 | 35s_T7_promoter_constitutive (gRNA with U6 promoter) |
| Part 3 | OCS 3′ terminator + RB |
| Part 4 | 35s + T7 + gRNA with U6 promoter (promoter banana) |
| Cas9 | Bba_K1218011 |
LB = left border, RB = Right border, U6: RNA polymerase III; T7: expression—gRNA, OCS: terminator.