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. 2024 Dec 19;14(12):627. doi: 10.3390/bios14120627

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mapping of recognition sites on DNA. (a) Single dCas9 probes bound to 3.6 kbp DNA translocating through a nanopore. (b) Double and triple dCas9 probe barcodes on full-length λ-DNA. (i) Example events with two and three peaks due to binding of the double-probe barcode and triple-probe barcode, respectively. (ii) Raw data comparing the number of peaks counted per event after the addition of a single probe, double probe, and triple probe. Reprinted with permission from [117]. Copyright 2019, American Chemical Society.