Figure 5.

Stability of endogenous TDP-43 in plasma and serum across freeze–thaw cycles. The bar plots represent the freeze–thaw stability of TDP43 across four freeze–thaw cycles of plasma (A) and serum (B). The results show minimal impact on assay performance and TDP-43 protein concentrations across the first two or three cycles. A modest reduction in TDP-43 concentration was noted in the fourth freeze–thaw cycle for plasma and the third or fourth freeze–thaw cycle for serum. The bars represent the average of replicate samples for each sample, with error bars representing +/− SD for each sample.