Table 7.
Partition of lipids in the different classes in SF of FO after digestion with or without capsules, evaluated by 1H-NMR.
| Not Digested | SF (Digested w/o Capsule) | SF (Digested with Capsule) | p-Value | |
|---|---|---|---|---|
| TAGs | 68.74 ± 0.16 a | 19.02 ± 7.76 b (19.02%) | 15.45 ± 0.05 b (24.35%) | <0.001 |
| 1,3-DAGs | 1.43 ± 0.01 a | 2.21 ± 0.81 a (2.2%) | 1.07 ± 0.42 a (1.7%) | 0.14 |
| 1,2-DAGs | 2.61 ± 0.02 b | 7.75 ± 2.22 a (7.75%) | 5.41 ± 0.66 a,b (8.54%) | 0.02 |
| 1,2-DAGs-P | n.d. | n.d. | n.d. | |
| 2-MAGs | 0.40 ± 0.02 b | 3.62 ± 1.21 a (3.62%) | 2.31 ± 0.71 a,b (3.67%) | 0.02 |
| 1-MAGs | 0.50 ± 0.02 b | 2.90 ± 1.39 a (2.09%) | 2.13 ± 0.45 a,b (3.35%) | 0.05 |
| WEs | 0.02 ± 0.001 ** | n.d. | 0.01 ± 0.002 (0.02%) | 0.0045 |
| Primary alcohol | 0.11 ± 0.01 a | 0.12 ± 0.08 a (0.12%) | 0.04 ± 0.03 a (0.07%) | 0.24 |
| Total P-choline | 0.04 ± 0.005 | n.d. | n.d. | |
| FFAs (except DHA) | 21.04 ± 0.14 b | 54.27 ± 11.92 a (54.27%) | 30.35 ± 5.38 b (47.62%) | 0.01 |
| Free DHA | 5.13 ± 0.05 b | 10.10 ± 1.15 a (10.10%) | 6.79 ± 0.28 b (10.68%) | 0.002 |
Data are expressed as mg/100 mg of not-digested oil and are means ± SD of two biological replicates, except for the not-digested oil, which was analyzed in three biological replicates. Each biological replicate was evaluated in triplicate. Statistical analysis was performed by Student’s t-test when comparing two values and by ANOVA (one-way analysis of variance) using Tukey’s test as post-test when comparing three values. p < 0.05 was considered significant. ** means p ≤ 0.01. Different letters in the same row indicate statistical significance. The mean percentage contribution of each class to total lipids in SF is reported in parentheses. TAGs = triglycerides; DAGs = diglycerides; MAGs = monoglycerides; WEs = wax esters; FFAs = free fatty acids; DHA = docosahexaenoic acid; FO = fish oil. n.d. = not detectable.