Table 11.
Partition of lipids in the different classes in SF of COs after digestion with or without capsules, evaluated by 1H-NMR.
| CO-1 | Not Digested | SF (Digested w/o Capsule) | SF (Digested with Capsule) | p-Value |
| TAGs | 0.91 ± 0.06 a | 0.51 ± 0.09 b (0.82%) | 0.50 ± 0.05 b (0.92%) | 0.004 |
| 1,3-DAGs | 0.06 ± 0.01 a | 0.03 ± 0.004 b (0.06%) | 0.03 ± 0.004 b (0.05%) | 0.02 |
| 1,2-DAGs | 1.16 ± 0.30 a | 1.14 ± 0.60 a (1.84%) | 0.63 ± 0.05 a (1.17%) | 0.34 |
| 1,2-DAGs-P | n.d. | n.d. | n.d. | |
| 2-MAGs | <0.01 | <0.01 | <0.01 | |
| 1-MAGs | 1.18 ± 0.01 a | 0.74 ± 0.03 b (1.20%) | 0.65 ± 0.06 b (1.20%) | <0.001 |
| WEs | 63.86 ± 0.59 a | 38.83 ± 0.33 b (62.64%) | 34.61 ± 2.79 b (64.13%) | <0.001 |
| Primary alcohol | 3.04 ± 0.45 a | 2.28 ± 0.24 a,b (3.67%) | 1.54 ± 0.03 b (2.86%) | 0.02 |
| Total P-choline | n.d. | n.d. | n.d. | |
| FFAs (except DHA) | 24.25 ± 0.15 a | 14.95 ± 0.24 b (24.12%) | 12.99 ± 0.85 c (24.09%) | <0.001 |
| Free DHA | 5.52 ± 0.03 a | 3.49 ± 0.08 b (5.63%) | 3.00 ± 0.26 b (5.56%) | <0.001 |
| CO-2 | Not Digested | SF (Digested w/o Capsule) | SF (Digested with Capsule) | p-Value |
| TAGs | 1.26 ± 0.06 a | 0.25 ± 0.07 b (0.48%) | 0.35 ± 0.02 b (0.66%) | <0.001 |
| 1,3-DAGs | 0.44 ± 0.03 a | 0.03 ± 0.01 b (0.05%) | 0.03 ± 0.01 b (0.05%) | <0.001 |
| 1,2-DAGs | 0.17 ± 0.10 a | 0.43 ± 0.21 a (0.81%) | 0.34 ± 0.21 a (0.63%) | 0.25 |
| 1,2-DAGs-P | n.d. | n.d. | n.d. | |
| 2-MAGs | 0.05 ± 0.01 | n.d. | n.d. | |
| 1-MAGs | 0.14 ± 0.002 b | 0.58 ± 0.07 a (1.08%) | 0.37 ± 0.26 a,b (0.70%) | 0.06 |
| WEs | 70.30 ± 0.03 a | 34.42 ± 0.59 b (64.71%) | 34.95 ± 0.09 b (65.70%) | <0.001 |
| Primary alcohol | 2.12 ± 0.03 a | 1.87 ± 0.26 a (3.50%) | 1.75 ± 0.14 a (3.29%) | 0.11 |
| Total P-choline | n.d. | n.d. | n.d. | |
| FFAs (except DHA) | 19.06 ± 0.08 a | 12.84 ± 1.50 b (23.94%) | 12.60 ± 0.69 b (23.68%) | 0.001 |
| Free DHA | 6.45 ± 0.10 a | 2.82 ± 0.17 b (5.30%) | 2.81 ± 0.03 b (5.29%) | <0.001 |
Data are expressed as mg/100 mg of not-digested oil and are means ± SD of two biological replicates, except for the not-digested oil, which was analyzed in three biological replicates. Each biological replicate was evaluated in triplicate. Statistical analysis was by one-way ANOVA using Tukey’s test as post-test, and p < 0.05 was considered significant. Different letters in the same row indicate statistical significance. The mean percentage contribution of each class to total lipids in SF is reported in parentheses. TAGs = triglycerides; DAGs = diglycerides; MAGs = monoglycerides; WEs = wax esters; FFAs = free fatty acids; DHA = docosahexaenoic acid; KO = krill oil. n.d. = not detectable.