Skip to main content
. 2005 Jul;17(7):1979–1993. doi: 10.1105/tpc.105.031096

Figure 4.

Figure 4.

Effects of BL and 1-NAA on the Expression of Integrated Reporter Genes and Natural Genes in Wild-Type Protoplasts or in nph4-1 Protoplasts Transfected with 35S:ARF7.

(A) Protoplasts were isolated from wild-type leaves containing an integrated DR5:GUS reporter gene and treated with indicated concentrations of BL or 1-NAA for 20 to 22 h, then GUS activities were measured. Standard errors are indicated. In some cases, error bars are not visible because of the small size of the error bars.

(B) Same as in (A), except protoplasts were isolated from wild-type leaves with an integrated 2XD0:GUS reporter gene.

(C) Same as in (A), except protoplasts were isolated from nph4-1 leaves containing an integrated DR5:GUS reporter gene and transfected with a 35S:ARF7 effector gene (10 μg).

(D) Same as in (A), except protoplasts were isolated from nph4-1 leaves containing an integrated 2XD0:GUS reporter gene and transfected with a 35S:ARF7 effector gene (10 μg).

(E) Protoplasts were isolated from wild-type leaves containing an integrated DR5:GUS or 2XD0:GUS reporter gene and treated with the concentration of BL or 1-NAA indicated for 20 to 22 h. Total RNA was isolated, and RT-PCR was used to test the expression levels of integrated DR5:GUS and 2XD0:GUS reporter genes and the natural auxin response genes IAA1, IAA19, GH3-2, and GH3-4. Expression of the natural ACT7 gene was used as a control.