Figure 3.

Expression of RAB-E1^d[NI] Does Not Directly Affect ER-to-Golgi Trafficking.

(A) to (C) Confocal images of a tobacco epidermal cell expressing secGFP (A) and YFP-HDEL (B) in presence of RAB-E1^d[NI]. (C) was obtained by merging images (A) and (B). Note that the punctate compartments labeled by secGFP fluorescence (arrows) are not marked by YFP-HDEL fluorescence. Note also the existence of a second type of punctate structure that is faintly labeled by secGFP but excludes YFP-HDEL (arrowheads in [C]). Bar in (C) = 10 μm for (A) to (C).

(D) Enlarged image of the confocal image in (C) showing details of small but bright (arrows) and apparently larger but faint (arrowheads) punctate structures labeled by secGFP but not by YFP-HDEL in the presence of RAB-E1^d[NI].

(E) and (F) Merged GFP/YFP confocal images of tobacco leaf epidermal cells expressing GFP-HDEL + YFP-HDEL in the presence of RAB-E1^d[NI] (E) and secGFP + YFP-HDEL in the presence of RAB-D2^a[NI] (F). Bar in (F) = 5 μm for (D) to (F).

(G) to (I) Confocal images of tobacco leaf epidermal cells expressing ST-YFP (G), ST-YFP + RAB-E1^d[NI] (H), and ST-YFP + RAB-D2^a[NI] (I). Bar in (I) = 10 μm for (G) to (I).