Figure 8.

Adenylate and Uridinylate Levels after Incubation of Tuber Discs Excised from Wild-Type Lines and UMPS Antisense Lines B33-8, B33-73, and B33-76 in 10 mM Orotate, Uridine, or Sucrose.

Discs were cut from developing tubers of 10-week-old plants and washed three times in buffer and were then incubated in 10 mM Mes-KOH, pH 6.5, containing 10 mM uridine (U), orotate (O), or sucrose (S). The discs were incubated for 2 h, washed three times, and extracted in trichloroacetic acid, and the levels of nucleotide and nucleoside were determined by HPLC. Data are presented as means ± se of determinations on six individual plants per line. Values marked with asterisks were determined to be significantly different from the time 0 control by t test.