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. 2024 Dec 17;13(24):4069. doi: 10.3390/foods13244069

Table 4.

Evaluation summary.

DNA Extraction Protocol LoD50 RLOD N PA NA PD ND SE SP AC k
TL 1.8 1.3 29 22 6 0 1 95.7 100.0 96.6 0.90
Beads 4.2 2.9 30 24 4 0 2 92.3 100.0 93.3 0.76
Chelex 2.2 1.5 27 21 5 0 1 95.5 100.0 96.3 0.89
GM 4.3 3.0 24 18 4 0 2 90.0 100.0 91.7 0.75

“TL”: thermal lysis. “GM”: thermal lysis with glass milk purification. LoD50: Limit of Detection 50%. RLOD: Relative Limit of Detection, with respect to ISO 6579-1:2017. PA, NA, PD and ND are “Positive Agreement”, “Negative Agreement”, “Positive Deviation” and “Negative Deviation”, respectively. SE, SP and AC are the relative sensitivity, specificity and accuracy, respectively, and the “k” is Cohen’s kappa value.