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. 2024 Dec 10;25(24):13258. doi: 10.3390/ijms252413258

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of the mutants. (A) Molecular size comparison between rTBL-1 and the mutants. SDS-PAGE of the electrophoretic profiles of rTBL-1, R103Q, and R130Q. Lane 1, ladder; lane 2, 5 µg of rTBL; lane 3, 5 µg of R103Q; lane 4, 5 µg of R130Q. (B) Analysis of glycosylation. Top panel shows a coomassie R-250-stained SDS-PAGE. N-Glycosidase F (PGNaseF), all O-glycosidases (β1,4-Galactosidase, endo-α-N-acetylgalactosaminidase, α2-3,6,8,9-Neuraminidase, and β-N-Acetylglucosaminidase), all N- and O-glycosidases (N-Glycosidase F, β1,4-Galactosidase, endo-α-N-acetylgalactosaminidase, α2-3,6,8,9-Neuraminidase, and β-N-Acetylglucosaminidase).