Figure 3.

Cytotoxic effect of rTBL-1 and its mutants on HT-29 colon cancer cells. Cells were treated with rTBL-1, R103Q, or R130Q (0, 0.25, 0.5, 2.5, 5, 25, and 50 μg/mL) for 24 h and cell survival percentages were calculated from hemocytometer counts. Small letters show significant differences for each separate treatment at different concentrations (Tukey p < 0.05), where differences between treatments for rTBL-1 were pointed out with letters a–e, for R103Q with letters f–h, and for R130Q with letter i.