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. 2024 Dec 16;25(24):13475. doi: 10.3390/ijms252413475

Figure 1.

Figure 1

Fermented grape extracts increased the efficacy of CPT treatment. (A) HeLa cells were treated with CPT at the indicated concentrations and incubated for 72 h. Cell viability was assessed using a standard assay. Statistical analysis was performed using a one-way repeated measures ANOVA with a multiple comparison post-test to compare all groups. Statistically significant differences are indicated as * p < 0.05 and **** p < 0.0001. (B) HeLa cells were treated with EtOAc extract in combination with DMSO and incubated as described in (A). *** p < 0.001 and **** p < 0.0001. (C) HeLa cells were treated with 2.5 nM CPT and various concentrations of EtOAc extract for 72 h. Cell viability was measured using assay. **** p < 0.0001. (D) IC50 values for EtOAc + DMSO and EtOAc + CPT were calculated using Prism 9 software. (E) HeLa cells were pre-treated with 0.63 μg/mL of EtOAc extract for one hour, followed by incubation with 1 μM CPT or 20 μM Etoposide for an additional two hours. Cells were then lysed, and Western blot analysis was performed using the indicated antibodies. Lamin A/C was used as a protein loading control.