Figure 2.

Multiple sequence alignment of CsILP1 and CsILP2 from female C. septempunctata with other insulin superfamily proteins. Sequences TcILP1, TcILP2b, TcILP3, and TcILP4 were derived from T. castaneum. Sequences DmILP1, DmILP2b, DmILP3, DmILP4, DmILP5, DmILP6, DmILP7, and DmILP8 were derived from D. melanogaster. The sequence name was preceded by GenBank accession number. Sequence Homo sapiens insulin was derived from humans. The canonical cleavage sites of the C-peptide are marked by black arrows. The common sequence is highlighted in red font. The green numbers represent the sites of the disulfide bonds, and the same numbers indicate they will be connected together after the cleavage of the C peptide.