| Strain, strain background (Escherichia coli) |
BL21(DE3) |
BioRad |
156–3003 |
Electrocompetent cells |
| Cell line (Homo-sapiens) |
HEK-293T epithelial-like cells |
ATCC |
CRL-3216 |
Cell line authentication services (STR profiling) are offered by ATCC, not detected mycoplasma contamination |
| Transfected construct (human) |
α-syn, Δ30 α-syn to pET22 vector |
This paper; Zhao et al., 2024
|
|
Constructs saved in C. Liu lab |
| Commercial assay or kit |
Neon transfection system kit |
Invitrogen |
MPK5000 |
|
| Chemical compound, drug |
16:0 LPC, DOPC, DOPS, POPC, POPE, biotin-DPPE, cholesterol |
Avanti Polar Lipids |
855675, 850375, 840035, 850457, 850757, 870277, 70000 |
|
| Chemical compound, drug |
Disuccinimidyl suberate (DSS) |
Thermo Scientific |
21658 |
|
| Chemical compound, drug |
uranyl acetate |
Sigma Aldrich |
CDS021290 |
|
| Chemical compound, drug |
DiD |
Invitrogen |
D307 |
|
| Software, algorithm |
pLink |
pLink |
V1.9 |
For XL-MS data |
| Software, algorithm |
SPARKY |
SPARKY |
V3.115 |
For NMR data |
| Software, algorithm |
NMRpipe |
NMRpipe |
Build2018 |
For NMR data |
| Software, algorithm |
smCamera |
TJ Ha’s lab |
|
For single vesicle data |
| Software, algorithm |
Dynamics |
Wyatt |
V7.0 |
For DLS data |
| Other |
C57BL6 mice |
Lingchang Shanghai |
8 wk old, male |
|
