Figure 5.

The deletion of UeMsb2 in U. esculenta resulted in significantly reduced expression of b signaling pathway genes during in vitro mating (A) and infection processes (B). Prf1 is essential for cell mating and hyphal growth [43]; bE1 and bW1 are involved in maintaining cell cycle arrest, filamentous hyphal growth, and subsequent infection processes [18]; Rbf1 plays a role in regulating filamentous growth, mating, and pathogenicity [15]; Hdp1, Biz1, and Kpp6 are responsible for regulating G2 cell cycle arrest, appressorium formation, and penetration of the host plant cell wall, respectively [15,44]; Clp1 is involved in regulating fungal development, pathogenicity, and fungal homeostasis [45]. β-actin was used as the internal reference gene. The data were analyzed using two-way ANOVA. Different lowercase letters indicate significant differences (p < 0.05), n = 3.