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Curves of the binding of rGmolPBP2 (wild-type) and its five mutants to Z8-14:Ac. The fluorescence intensity after Z8-14:Ac displaced the 1–NPN probe bound to rGmolPBP2 is displayed as a percentage of the initial fluorescence intensity. The calculated Ki values of rGmolPBP2 (wild-type) and mutants with Z8-14:Ac are listed in Table 5.
