Table 5.
Binding affinities of wild-type and mutants of GmolPBP2 to Z8-14:Ac.
| Protein Name | IC50 (μM) | Ki (μM) |
|---|---|---|
| wild-type | 1.260 ± 0.036 | 0.660 ± 0.019 |
| F12A | >14 | >14 |
| L68A | 1.129 ± 0.026 | 0.589 ± 0.007 |
| I94A | 1.082 ± 0.008 | 0.564 ± 0.004 |
| R109A | 1.257 ± 0.029 | 0.655 ± 0.015 |
| I113A | 0.980 ± 0.038 | 0.520 ± 0.020 |
Note: IC50: the concentration of Z8-14:Ac when replacing 1–NPN to reduce the initial fluorescence intensity of rGmolPBP2 (and its mutants)/1–NPN complex to 50%; Ki: the inhibition constants of Z8-14:Ac competitive binding with wild-type GmolPBP2 and each mutant from the protein/1–NPN complex. Data in the table are presented as mean ± SE (n = 3).