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. 2024 Dec 12;16(12):536. doi: 10.3390/toxins16120536

Table 3.

Summary of the experimental setup: objectives and experimental conditions.

Set of Experiment Objective Experimental Conditions
1 Evaluate the effect of the H2O2/Fe(II) molar ratio on CYN degradation H2O2: 75 µM and Fe(II): 22.1 to 375.0 µM;
H2O2/Fe(II) molar ratio: 0.2, 0.4, 0.5, 0.6, 0.8, 1.0, 1.6, and 3.4;
Initial pH: 5.0;
CYN: 0.05 µM and Matrix: Paranoá Lake water.
2 Assess the effect of H2O2 and Fe(II) dosages on CYN degradation H2O2: 25 to 100 µM and Fe(II): 62.5 to 250.0 µM;
Optimum H2O2/Fe(II) molar ratio from set 1;
Initial pH: 5.0;
CYN: 0.05 µM and Matrix: Paranoá Lake water.
3 Examine the effect of initial CYN concentration on oxidation efficiency H2O2 and Fe(II) concentrations from set 1;
Optimum H2O2/Fe(II) molar ratio from set 1;
Initial pH: 5.0;
CYN: 0.05, 0.1 and 0.2 µM and Matrix: Paranoá Lake water.
4 Evaluate the effect of initial pH on CYN degradation H2O2 and Fe(II) concentrations from set 1;
Optimum H2O2/Fe(II) molar ratio from set 1;
Initial pH values: 3, 4, 5, 7, 9;
CYN: 0.05 µM and Matrix: Paranoá Lake water.
5 Examine the effect of humic acid (HA) and algogenic organic (AOM) matter on CYN degradation 5.1 H2O2: 25 to 100 µM and Fe(II): 62.5 to 250.0 µM;
Optimum H2O2/Fe(II) molar ratio from set 1;
Initial pH: 5.0;
CYN: 0.05 µM and Matrix: ultrapure water.
5.2 H2O2: 25 to 100 µM and Fe(II): 62.5 to 250.0 µM;
Optimum H2O2/Fe(II) molar ratio from set 1;
Initial pH: 5.0;
HA concentration: 5 mg/L;
CYN: 0.05 µM and Matrix: ultrapure water.
5.3 H2O2: 25 to 100 µM and Fe(II): 62.5 to 250.0 µM;
Optimum H2O2/Fe(II) molar ratio from set 1;
Initial pH: 5.0;
CYN: 0.05 µM from Raphidiopsis raciborskii crude extract and Matrix: ultrapure water.
6 Compare the degradation of CYN in methanol–free ultrapure water and ultrapure water containing methanol 6.1 H2O2: 25 µM and Fe(II): 7.4 to 125.0 µM;
H2O2/Fe(II) molar ratio: 0.2, 0.4, 0.5, 0.6, 0.8, 1.0, 1.6, and 3.4;
Methanol: 0 and 513.3 µM;
Initial pH: 5.0;
CYN: 0.05 µM and Matrix: ultrapure water.
6.2 H2O2: 10 to 50 µM and Fe(II): 25 to 125 µM;
Optimum H2O2/Fe(II) molar ratio from set 6.1;
Methanol: 0 and 513.3 µM;
Initial pH: 5.0;
CYN: 0.05 µM and Matrix: ultrapure water.