Figure 1.

Schematic representation of the genetic constructs expressing the C. amphilecti KMM 296 alp gene encoding rCmAP in N. tabacum. The constructs feature the 35S CaMV promoter driving CmAP expression. Key elements include the NOS promoter and terminator for regulatory control, NPTII as a selectable marker, and left (LB) and right (RB) border regions for Agrobacterium-mediated plant transformation. Additional components include viral suppressors of RNA silencing (p24 and NS), a subgenomic promoter region (SGP), and an RNA-dependent RNA polymerase (RDRP) for RNA replication. Both constructs include a 6×His tag and an enterokinase cleavage site for simplified purification.