Table 2.
Optimized conditions for LC-MS quantification of PFOS and PFOA
| LC | MS | ||
|---|---|---|---|
| Instrument | Agilent 1100 Series | Instrument: atmospheric pressure electrospray ionization (ESI) MS detector (Agilent Technologies, USA) | |
| Column | ODS-C18 ((2.1 × 150 mm, 5 mm), Waters, USA | ||
| Mobile phase |
A: CH3CN B: CH3COONH4/H2O (10mM) |
Polarity mode | Negative |
| Elution | Linear gradient: started at 35% of A and then increased to 45% of A at 2%/min for 5 min and maintained at 45% untile the end of run time | Nebulizer | N2 (70 psi) |
| Drying gas | N2 (10 L/min, 350 °C) | ||
| Fragmenter voltage | 130 V | ||
| capillary voltage | 4000 V | ||
| Flow rate | 0.2 ml/min | m/z (SIM mode) |
499 for PFOS 413 for PFOA |
| Oven temperature | Room temperature | ||
| Injection volume | 20 µL | Data processing | ChemStation software |
| Run time | 20 min | ||