Fig. 2.

Multiomic signature of SLAMF7 expressing T cells. (A) Poly-functionality of SLAMF7⁺ T cells. Functional heterogeneity of T cells revealed by Flow Cytometry analysis after PMA/Ionomycin stimulation. Poly-functionality of T cells was analyzed by SPICE and compared SLAMF7⁺ with SLAMF7^- CD4 (CD154⁺) and CD8 (CD154^-) T cells. The pies represented the frequencies of T cells with a defined combination of effector molecules. The arcs above the pie indicate which cytokines or cytolytic molecules are expressed by each slice of the pie. (B) Effector signature of SLAMF7⁺ T cells during aging. Effector Memory (CD45RO⁺CD27^-) T cells from young (n = 5) and older donors (n = 3) were sorted and stimulated overnight with PMA/Ionomycin. Supernatants were analyzed by Luminex and visualized by PCA. The contribution of individual cytokines to the main components was also indicated. (C) Effector signature of SLAMF7⁺ T cells during aging. Sorted SLAMF7⁺ T cell subsets were directly lysed ex-vivo. Gene expression was quantified by customized senescence Nanostring, normalized by Z-score calculation, and represented by a cold-to-hot heat map. (D) Transcriptional signature of SLAMF7⁺ T cells during aging. Top50 of differentially expressed genes between SLAMF7-expressing EM T cells was visualized by PCA. The contribution of individual genes to the main principal components was also indicated. (E) Exhaustion and transcription factors profile of SLAMF7⁺ T cells during aging. The SLAMF7-specific gene expression assessed as in (D), was represented by a cold to hot heat map for genes associated with activation/inhibitory molecules (left) and TF/signaling pathways (right).