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. 2024 Dec 28;14:30779. doi: 10.1038/s41598-024-80971-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — SLAMF7 signaling during aging. (A) SLAM signaling pathway in CMV-specific CD8 T cells. RNA Sequencing was used to evaluate the mRNA expression of constituent molecules of this pathway. The scaled gene expression intensity was visualized by a cold-to-hot heat map. (B) Modulation of the SLAM signaling pathway in CMV-specific CD8 T cells during aging. The gene expression was quantified by bulk RNA Sequencing in total or CD27 subsets of CMV-specific CD8 T cells from young or older donors (n = 10 and n = 3 respectively) and expressed as transcript per Million. (C) STAT_1/3 signaling pathway in CMV-specific CD8 T cells. Flow cytometry was used to evaluate the constitutive (unstimulated) and IFNγ/IL-6-induced phosphorylation of STAT1 and STAT3 in CMV-specific CD8 T cells (red) and total CD8 T cells (grey) from young and older donors. Dot plots from four representative patients are described. (D) Quantification of STAT_1/3 phosphorylation in CMV-specific CD8 T cells. The combination of phosphoSTAT1 (pSTAT₁) and pSTAT₃ was compared between total and CMV-specific CD8 T cells, left. The co-expression of pSTAT₁ with phenotypic markers such as SLAMF7, CD27, GPR56, and CD126 was evaluated for total and CMV-specific CD8 T cells, right. The statistical analysis was performed on paired samples (n = 16, Wilcoxon signed-rank test), (*, **, and **** for p < 0.05, p < 0.01, and p < 0.0001, respectively). (E) SLAMF7 and STAT₁ signaling in CMV-specific CD8 T cells. The pSTAT₁ and pSTAT₃ were evaluated after SLAMF7 ligation in CMV-specific CD8 T cells. The co-expression of pSTAT₁ and pSTAT₃ or pSTAT₁ with phenotypic markers were analyzed as in (D).