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. 2024 Dec 28;14:31001. doi: 10.1038/s41598-024-82165-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Effect of kM-RIIIJ on locomotion of mice. (A). Assay setup. Animals were given a daily i.p. injection of saline solution. 30 min post injection, animals were placed in a chamber with a running wheel coupled to an electromagnetic odometer. Animals were trained on running wheel for 4 days. On day 5, animals were injected with either saline or kM-RIIIJ 30 min prior final trial. (B). Normalized percentage of activity. The number of turns on day 4 was considered as 100%. The toxin injected group was compared to the controls using a multiple unpaired t test. ***** p < 0.00001. ns = not significant.