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Construction of an inducible secretory expression system, pKS81. (A) The sequence of the HptR binding site, uhpT promoter (-35 and -10), ribosome binding site, and N-terminal signal peptide sequence of the Hlb, multicloning sites, and 8 × histidine tag cloned into the pOS1 plasmid. (B) A schematic illustration of recombinant protein expression, secretion, and purification process.
