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. 2024 Dec 28;14:30780. doi: 10.1038/s41598-024-81001-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — Enhanced expression and purification of human annexin A2 tagged with mIgG1 by an inducible and secretory protein expression vector system, pKS81. (A) Expression of AnxA2-mIgG1in pET expression system using a pET28 vector in E. coli BL21 (DE3) pLysS strains with IPTG induction. Protein expression was analyzed by the SDS-PAGE with Coomassie Blue staining. M: Protein marker, lane 1: Uninduced, lane 2: IPTG induced soluble fraction, lane 3: IPTG induced insoluble fraction. (B) Expression of AnxA2-mIgG1 using a pKS81vector in S. aureus LAC9. Transformed S. aureus LAC9 was cultured in the CY broth with or without G6P (2%, w/v) for 18 h at 37^o C with shaking at 200 rpm. The culture supernatant was collected and AnxA2-mIgG1 was purified by the nickel affinity chromatography. Protein expression and purification were analyzed by the SDS-PAGE with Coomassie Blue staining. M: Protein marker, lane 1: culture supernatant from CY broth without G6P, lane 2: culture supernatant from CY broth with G6P, lane 3: purified human annexin A2 with mIgG1 tag.