Table 2.
List of antibodies used to study corneal endothelial cells on flat-mounted whole corneas.
| Target protein | Source | Isotype | Laboratory | Reference | Fixation | Secondary antibodies |
|---|---|---|---|---|---|---|
| Anti-YAP1 (63.7) | Mouse | IgG2a |
Santa Cruz Biotechnology, Inc. Germany |
sc-101199 | Methanol | Goat anti-mouse IgG, Alexa Fluor™ 488, A32723, Invitrogen |
| Anti-phospho-YAP1, p-YAP; D9W2I (ser127) | Rabbit | / | Cell signaling Technology, Danvers MA | 13008S | PFA | Goat anti-rabbit IgG, Alexa Fluor™ 488, A11034, Invitrogen |
| Anti-ZO-1 | Rabbit | / | ThermoFisher Scientific Inc., USA | 40-2200 |
Methanol PFA |
Goat anti-rabbit IgG, Alexa Fluor™ 555, A21428, Invitrogen |
| Mouse | IgG1 | BD Bioscience, Belgium | 610967 | PFA |
1. Goat anti-mouse IgG, Alexa Fluor™ 555, A11003, Invitrogen 2. Goat anti-mouse IgG1, Alexa Fluor™ 633, A21126, Invitrogen |
|
| Anti-acetylated α tubulin (6-11 B-1) | Mouse | IgG2b | Santa Cruz | sc-23950 | PFA |
1. Goat anti-mouse IgG, Alexa Fluor™ 555, A11003, Invitrogen 2. Goat anti-mouse IgG2b, Alexa Fluor™ 555, A21147, Invitrogen |