Figure 5. Ezetimibe alleviated PD‐induced liver steatohepatitis and hypercholesterolemia.

Liver sections, serum, and liver homogenate were collected from mice in the ND‐fed vehicle control mice group (PBS+DMSO), the 8‐weeks PD‐fed vehicle control group (PBS+DMSO) and the PD+EZE treatment group (I.P. 3 mg/kg per 2 days for 8 weeks). Liver histomorphology was measured using H&E staining (A). Liver lipid deposition was detected by oil Red O staining (B and E) and lipid droplet–associated protein perilipin 2 staining (C and F). Liver inflammatory cell infiltration was indicated by leukocyte marker CD45 staining (D and G). TC (H and I) and TG (J and K) levels in serum and liver homogenate were measured. Scale bar=20 μm. * vs ND, # vs PD, P<0.05 (n=6–8). EZE indicates ezetimibe; H&E, hematoxylin and eosin; ND, normal diet; PD, Paigen's diet; TC, total cholesterol; and TG, triglyceride.