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. 2024 Dec 28;24:544. doi: 10.1186/s12866-024-03715-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — Relative mRNA expressions of virulence genes and oxidative stress response genes by RT-qPCR in CE1/pSTV28, CE1ΔybdO/pSTV28, CE1ΔybdO/pCybdO, CE1/pUC19, and CE1/pUCybdO. (A) Relative transcription levels of fepG, ycgV, sodA, soxR, ahpC, ahpF, katG, and oxyR were determined by RT-qPCR in CE1/pSTV28, CE1ΔybdO/pSTV28, and CE1ΔybdO/pCybdO cultured in LB broth with 16 μg/mL chloramphenicol. (B) Relative transcription levels of fepG, ycgV, sodA, soxR, ahpC, ahpF, katG, and oxyR were determined by RT-qPCR in CE1/pUC19 and CE1/pUCybdO cultured in LB broth with 100 μg/mL ampicillin. Error bars indicate standard deviations. The relative gene expressions were calculated using the 2^−ΔΔCt method. **P < 0.01, indicating the extremely significant difference; *P < 0.05, indicating the significant difference