Fig. 4.

The binding ability of YbdO to target gene promoters was determined by EMSA. Increasing amounts of YbdO were incubated with Biotin-labeled kpsM, fepG ycgV, sodA, soxR, ahpC, katG, oxyR, and lacZ (Biotin-kpsM, Biotin-fepG Biotin-ycgV, Biotin-sodA, Biotin-soxR, Biotin-ahpC, Biotin-katG, Biotin-oxyR, and Biotin-lacZ). In each panel, from lanes 1 to 5, the concentrations of YbdO were 8, 0, 2, 4 and 8 μM, respectively; the amount of Biotin-labeled probes in all lanes was 100 fmol. In lane 1, besides the labeled probes, 1 pmol of unlabeled probe was added as the competitive control (Ctrl). (A) The positive control, the binding ability of YbdO to the kpsM promoter; (B) the fepG promoter; (C) the ycgV promoter; (D) the sodA promoter; (E) the soxR promoter; (F) the ahpC promoter; (G) the katG promoter; (H) the oxyR promoter; (I) The negative control, the binding ability of YbdO to the lacZ encoding DNA fragment