Fig. 5.

The Personalized Functional Profiling (PFP) technology. Surgical patient biopsies are dissociated and culturing tumor spheroid. Dissociated cells are printed onto a 3D-pillar support system using robot technology and grown as tumor spheroids. These tumor spheroids have the same genomic composition as the tumor of origin. Tumor spheroids are then challenged by a large panel of drugs (including FDA approved drugs). Growth (size of spheroids) is measured by an automated confocal microscope. To foster a comparative analysis of drug responses, we will employ an analysis of drug sensitivity, including the half-maximal inhibitory concentration (IC₅₀) and the area under curve (AUC) of the dose–response curve (DRC). Drugs that show efficacy can potentially be reported to patients