Fig. 9.

A Diagram of 3D extrusion based bioprinter working process: biomaterial, populations of interest and biomolecules are inserted into the instrument and extruded together in a software-aided shape. B Immunohistochemistry analysis conducted on 3D OS bioprinted models showing the positivity for Vimentin (magnification 100X). C 3D OS models were bioprinted starting from Sa-Os cell line alone (on the left) or in combination with fibroblasts (on the right). The models were cultured in a 6-well chamber slide and stained with the live-dead assay kit (Merck). The rows show live cell OS models stained with (a, e, i) Calcein-AM, (b, f, j) propidium iodide, (c,g,k) Hoechst 33,342 and (d,h,l) merged image