Abstract
The first detailed results of the application of a low-resolution mixture analysis approach to the sequence analysis of an enzyme, ribitol dehydrogenase, are given. Examples of the interpretation of the spectra of peptide mixtures derived from this protein are described. Evidence for new fragmentation patterns observed is reported, together with an explanation of the generation of ambiguous sequences by use of a low-specificity enzyme, thermolysin. The overall sequencing strategy evolved is assessed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Agarwal K. L., Johnstone R. A., Kenner G. W., Millington D. S., Sheppard R. C. Mass spectrometry of N-methylated peptide derivatives. Nature. 1968 Aug 3;219(5153):498–499. doi: 10.1038/219498a0. [DOI] [PubMed] [Google Scholar]
- Lucas F., Barber M., Wolstenholme W. A., Geddes A. J., Graham G. N., Morris H. R. Mass-spectrometric determination of the amino acid sequences in peptides isolated from the protein silk fibroin of Bombyx mori. Biochem J. 1969 Oct;114(4):695–702. doi: 10.1042/bj1140695. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris H. R., Batley K. E., Harding N. C., Bjur R. A., Dann J. G., King R. W. Dihydrofolate reductase: low-resolution mass-spectrometric analysis of an elastase digest as a sequencing tool. Biochem J. 1974 Feb;137(2):409–411. doi: 10.1042/bj1370409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris H. R., Dickinson R. J., Williams D. H. Studies towards the complete sequence determination of proteins by mass spectrometry: derivatisation of methionine, cysteine and arginine containing peptides. Biochem Biophys Res Commun. 1973 Mar 5;51(1):247–255. doi: 10.1016/0006-291x(73)90535-4. [DOI] [PubMed] [Google Scholar]
- Morris H. R. Studies towards the complete sequence determination of proteins by mass spectrometry; a rapid procedure for the successful permethylation of histidine containing peptides. FEBS Lett. 1972 May 15;22(3):257–260. doi: 10.1016/0014-5793(72)80244-8. [DOI] [PubMed] [Google Scholar]
- Morris H. R., Williams D. H., Ambler R. P. Determination of the sequences of protein-derived peptides and peptide mixtures by mass spectrometry. Biochem J. 1971 Nov;125(1):189–201. doi: 10.1042/bj1250189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roepstorff P., Spear R. K., Brunfeldt K. Mass spectrometric sequence determination of permethylated peptide mixtures. FEBS Lett. 1971 Jun 24;15(3):237–240. doi: 10.1016/0014-5793(71)80320-4. [DOI] [PubMed] [Google Scholar]
- Shemyakin M. M., Ovchinnikov Y. A., Kiryushkin A. A., Vinogradova E. I., Miroshnikov A. I., Alakhov Y. B., Lipkin V. M., Shvetsov Y. B., Wulfson N. S., Rosinov B. V. Mass spectrometric determination of the amino-acid sequence of peptides. Nature. 1966 Jul 23;211(5047):361–366. doi: 10.1038/211361a0. [DOI] [PubMed] [Google Scholar]
- Shotton D. M., Hartley B. S. Evidence for the amino acid sequence of porcine pancreatic elastase. Biochem J. 1973 Apr;131(4):643–675. doi: 10.1042/bj1310643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor S. S., Rigby P. W., Hartley B. S. Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure. Biochem J. 1974 Sep;141(3):693–700. doi: 10.1042/bj1410693. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wibf H. K., Irving P., McCamish M., Venkataraghavan R., McLafferty F. W. Mass spectrometric studies of peptides. V. Determination of amino acid sequences in peptide mixtures by mass spectrometry. J Am Chem Soc. 1973 May 16;95(10):3369–3375. doi: 10.1021/ja00791a048. [DOI] [PubMed] [Google Scholar]