Table 1.

Thermodynamic profiles of wildtype and mutant antibodies interacting with the phosphopeptides

Antibody	KD (nM)	ΔG (kcal/mol)	ΔH (kcal/mol)	TΔS (kcal/mol)
A4 WT	3.34 ± 0.63	−11.6 ± 0.1	−24.4 ± 0.4	−12.8 ± 0.4
A4 H-S53A	3.55 ± 1.72	−11.6 ± 0.3	−24.9 ± 0.5	−13.3 ± 0.3
A4 H-S54A	6.95 ± 2.32	−11.2 ± 0.2	−23.5 ± 0.2	−12.3 ± 0.4
A4 H-T56A	4.00 ± 0.89	−11.5 ± 0.1	−23.0 ± 0.6	−11.5 ± 0.6
A4 H-R95A	No binding
C7 WT	26.9 ± 14.2	−10.4 ± 0.4	−14.4 ± 0.7	−4.0 ± 1.0
C7 H-S52A	664 ± 34	−8.4 ± 0.0	−11.1 ± 0.1	−2.7 ± 0.1
C7 H-R53A	No binding
C7 H-S54A	95.3 ± 20.9	−9.6 ± 0.1	−13.3 ± 0.5	−3.7 ± 0.6