Table 2.
Precision of the NanoLuc bioluminescence assays on three separate days (E1, E2, E3). Three concentrations were determined by bioluminescence measurement. Data are presented as mean (± standard error). CFU colony-forming unit
Concentration (CFU/mL) | Determined concentration (CFU/mL) | Coefficient of variation (%) | ||||
---|---|---|---|---|---|---|
E1 | E2 | E3 | E1 | E2 | E3 | |
1.20 × 108 | 1.81 (± 0.06) × 108 | 1.27 (± 0.04) × 108 | 1.16 (± 0.01) × 108 | 7.47 | 8.48 | 2.04 |
7.00 × 107 | 6.93 (± 0.29) × 107 | 8.94 (± 0.28) × 107 | 8.11 (± 0.28) × 107 | 10.32 | 7.69 | 8.51 |
2.50 × 107 | 2.81 (± 0.21) × 107 | 2.51 (± 0.19) × 107 | 3.49 (± 0.12) × 107 | 18.05 | 18.98 | 8.24 |