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T cell responses and cytokine levels in the supernatant of splenic lymphocytes at day 28 in BALB/c mice. (a-b) IFN-γ ELISpot test following antigen-specific splenic stimulation in mice. (c) TNF-α ELISpot test following antigen-specific splenic stimulation in mice. (d) Multiplex cytokine analysis of splenic lymphocyte supernatant stimulated by peptides. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. N = 6.
