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. 2024 Dec 28;14:31327. doi: 10.1038/s41598-024-82793-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — IQGAP3 promoted the migration, invasion and stemness potential of lung cancer. (A) Down-regulation of IQGAP3 in H1299 and A549 cells after IQGAP3 siRNA transfection. original blots/gels are presented in Supplementary Fig. 3 .(B-C) Representative images and quantitative data of wound healing assays in H1299 and A549 cells after IQGAP3 down-regulation. Scale bar = 200 μm. (D-F) Representative images and quantitative data of Transwell migratory and invasive assays in H1299 and A549 cells after IQGAP3 down-regulation. Scale bar = 50 μm. (G-H) Sphere formation ability of lung cancer cells via sphere formation assays after silencing IQGAP3. Scale bar = 100 μm. Data are shown as mean ± SD from experiments in triplicate. **P < 0.01, ***P < 0.001, ns not significant.