Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Dec 28;14:31327. doi: 10.1038/s41598-024-82793-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 4 — IQGAP3 interacted with GLI1 of Hedgehog pathway in lung cancer. (A) Volcano plot showing the differentially expressed genes between the IQGAP3 down-regulated H1299 cells and control group according to RNA-sequencing. (B) Heatmap showing the reduction in GLI1 expression after the knockdown of IQGAP3 in H1299 cells. (C) Validation experiments via RT-qPCR confirming mRNA expression changes in Hedgehog pathway and EMT-associated genes in A549 and H1299 cells after IQGAP3 down-regulation. (D-E) Representative images and quantified data from western blotting validating protein expression changes in Hedgehog pathway and EMT-associated genes in A549 and H1299 cells following IQGAP3 down-regulation. original blots/gels are presented in Supplementary Fig. 3 .(F) Co-immunoprecipitation assays demonstrating the interaction between endogenous IQGAP3 and GLI1 in A549 and H1299 cells. original blots/gels are presented in Supplementary Fig. 3.